石油烃类污染物降解动力学和微生物群落多样性分析

为了探讨不同初始浓度石油污染土壤堆腐化修复机制,以石油降解菌剂和腐熟鸡粪为调理剂,研究了初始浓度分别为5 000(T1)、10 000(T2)和50 000 mg/kg(T3)的石油污染土壤堆腐化修复过程石油烃类污染物降解动力学特征和微生物群落多样性。结果表明:堆腐化修复过程石油烃类污染物降解符合一级反应动力学,反应常数分别为0.012、0.094和0.050 d-1,半衰期分别为6.79、7.37和13.86 d。整个堆腐过程石油烃类污染物平均降解速率分别为112.08、230.05和887.93 mg/(kg·d)。3个处理的孔平均颜色变化率(average well color development)和碳源利用率(除芳香烃类化合物外)随堆腐进程的推进逐渐升高,在堆腐中、后期达到最大,T3处理显著高于T1、T2处理。多聚物类和糖类代谢群是堆腐体系中的优势菌群。主成分分析表明3个处理的微生物群落差异显著(除第9天外),起分异作用的碳源主要是糖类和羧酸类。微生物群落的丰富度指数和均一度指数随堆腐进程的推进逐渐升高并在堆腐后期达到最大,与T1处理相比,T3处理分别高了0.21%和17.64%,差异达到显著水平(P0.05)。微生物群落优势度指数在中期达到最大,T1处理分别比T2、T3处理高2.12%和9.44%,3个处理间差异不显著(P0.05)。堆肥结束时3个处理的种子发芽指数(seed germination index,SGI)分别比堆腐初期提高了18.26%、20.42%和36.41%。该研究结果为黄土高原不同程度石油污染土壤堆腐化修复的应用提供参考依据和理论基础。     

Short- and long-term effects of heavy metals on phosphatase activity in soils: An ecological dose-response model approach

Summary The aim of this study was to provide manageable data to help establish permissible limits for the pollution of soil by heavy metals. Therefore the short-and long-term effects of heavy metal pollution on phosphatase activity was studied in five different soil types. The results are presented graphically as logistic dose-response curves. It was possible to construct a curve for sand and silty loam soil but it was more difficult to establish a curve for sandy loam and clay soil and nearly impossible (except for Cu) for peat. The toxicity of the various metals can be compared on the basis of mmol values. In clay soils, for Cd, Cr, Cu, and Zn, the 50% effective ecological dose (ED₅₀) values were comparable (approximately 45 mmol kg^–1), but the ED₁₀ values were very different, at 7.4, 41.4, 15.1, and 0.55, respectively. At the ED₅₀ value, toxicity did not decrease with time and, in sandy soils, was approximately 2.6 mmol kg ^–1 dry soil for Cd, Cu, and Zn. In four out of five soils, the Cd toxicity was higher 1.5 years after the addition of heavy metal salts than after 6 weeks. Toxicity was least in the sandy loam, silty loam, and clay soil, and varied in general between 12 and 88 mmol kg^–1. In setting limits, the criteria selected (no-effect level, ED₁₀ or ED₅₀) determine the concentration and also the toxicity of the sequence. It is suggested that the data presented here could be very useful in helping to set permissible limits for heavy metal soil pollution.     

Interrelationships among Coconut (Cocos Nucifera L.) Accessions Using RAPD Technique

The genetic relationships among 33 coconut germplasm accessions were analyzed using RAPD markers. The germplasm accessions were collected from various coconut growing regions viz. South Asia (SA), South East Asia (SEA), South Pacific (SP), Atantic and America, and Africa. Forty-five random primers produced a total of 399 polymorphic markers. The Polymorphism Information Content (PIC) ranged from 0.031 to 0.392 and the Marker Index (MI) ranged from 6.28 to 0.031 among the primers. Based on the MI a set of 5, 10 and 15 informative and reproducible primers were identified. The mantel matrix correlation was calculated to compare the similarity matrices of a set of reproducible informative primers and global primers. There was significant correlation among the similarity matrices (r ≥ 0.50). The similarity matrix based on 399 polymorphic markers was used to construct the dendrogram to show the genetic relationship among the accessions. Similarity values ranged between 0.573 and 0.846. There was less genetic similarity (based on Jaccard's coefficient) among South Pacific and South East Asian accessions. The clustering pattern obtained in the present study was in agreement with the earlier reports based on RFLP, SSRs and AFLPs.     

Long-term impacts of even-aged timber management on abundance and body condition of terrestrial amphibians in Northwestern California

Conservation needs for amphibians in managed timberlands may differ based upon the species present and the timber harvesting methods employed. Clearcuts have been documented to be detrimental to amphibians but the impacts of associated silvicultural edges and alternative harvesting treatments are not well understood. The primary objective of this study was to determine if amphibian abundances and body condition differed in thinned forests and intact forests, and in clearcuts and associated silvicultural edges. We also examined which environmental attributes were important in explaining observed differences. We sampled clearcuts, silvicultural edges, and adjacent late-seral forests at 10 sites in northwestern California from October 1999 to July 2002. Clearcuts at these sites ranged in age from 6 to 25 years. Five of these forest stands were intact and five had been commercially thinned at least 10 years prior to our study. Amphibian abundances were similar in thinned and unthinned forests, but body condition of the most common species was lower in thinned forests. Abundances of amphibians were nearly twice as high in forests and at silvicultural edges than in clearcuts. Clearcutting at these sites appears to have affected amphibian numbers up to 25 years post-harvest, however, silvicultural edges were suitable habitats for amphibians. While commercial thinning did not reduce amphibian numbers, it is an intermediate treatment followed by clearcutting. Where conservation of amphibians is a concern, even-aged silvicultural systems may not provide the most appropriate method for maintaining viable populations on managed forestlands in the northwestern US.     

鄱阳湖生态经济区畜禽养殖土壤环境承载力及污染风险研究

[目的]测算和评价鄱阳湖生态经济区畜禽养殖过程中的土壤环境承载力及其污染风险,为合理规划鄱阳湖生态经济区畜禽养殖空间布局、实现可持续养殖提供参考。[方法]利用2000—2012年鄱阳湖生态经济区24个县(市)的统计数据进行土壤环境承载力及畜禽污染物排放量的测算和评估。[结果]2000—2012年鄱阳湖生态经济区畜禽粪便耕地负荷警报值在0.7左右,总氮耕地负载警报值为0.5~0.6,总磷耕地负载警报值超过了1,畜禽养殖对环境造成了污染威胁。畜禽养殖污染风险较高的地区为高安市、东乡县、余江县、德安县以及南昌县。[结论]污染风险较高的地区应强制实行畜禽养殖总量控制和污染物消减措施,而湖口县、都昌县和彭泽县畜禽污染风险较低,可以适当扩大养殖规模。     

我国设施农业农膜使用的环境问题刍议

农膜使用和地面覆盖技术的应用加快了我国农业现代化的步伐,增加和丰富了农产品的供给。但随着农膜的连续使用,导致大量的农膜残留,严重影响农业生产,并对农业生态环境安全和人体健康构成巨大威胁。本文总结了设施农业中农膜使用所造成的主要环境问题,剖析了这些问题的内在原因。在此基础上,针对我国设施农业中农膜使用和管理现状,分别从管理、政策、经济和技术方面提出了对策建议,以期为规范我国设施农业中农膜使用、提高设施农业环境管理效率和健康持续发展提供理论依据。     

黄淮海平原县域农村居民点布局优化及其整治策略

为合理确定农村居民点的布局优化方向,科学指导土地整治和新农村建设中农村居民点的迁移合并实践,该文以河北省曲周县为例,从农村居民点自身发展条件和邻近居民点的空间相互作用关系2个角度,构建了以斑块为单元符合平原区特点的农村居民点结节性指数评价体系,利用多因素综合评价法测算了各农村居民点结节性指数值;利用引力模型确定了整理单元,总结出农村居民点间的4种空间相互作用关系,即串联型、并联型、聚合型、双核型;在此基础上,分别利用场强模型和引力模型将河北省曲周县242个主要农村居民点划分为城镇化型、重点发展型、规模控制型和迁移合并型,并有针对性的提出布局优化调控建议。研究结果符合曲周县实际情况,为当地农村居民点整理工作提供了依据,对黄淮海平原其他农区的农村居民点科学规划与布局有一定借鉴意义。     

森林凋落物分解研究进展

 森林凋落物分解是森林生态系统养分生物循环的重要环节,而分解过程中所释放的CO2是全球碳素收支的重要组分,开展森林凋落物分解研究是充分认识森林生态系统结构和功能的基础。研究认为:凋落物分解的预测指标可分为3类,即环境指标(如实际蒸散量)、凋落物物理质量(如叶抗张强度)和化学质量指标(如C/N比、木质素/N比和C/P比等);凋落物分解过程中养分释放机制极其复杂,养分动态模式主要有淋溶—释放、淋溶—富集—释放和富集—释放3种,并因凋落物种类、分解阶段和元素本身性质的不同而异;凋落物混合分解并非单一树种分解的简单叠加,因树种组成和比例不同,基质的化学组成会发生变化,从而影响分解者的多样性、丰富度和生理活性,进而直接和间接地影响其分解速率;凋落物混合分解中可能存在无效应、促进效应和抑制效应;现有的研究结果显示,凋落物混合分解的适宜比例应与群落中不同树种的种群比例相一致;CO2浓度升高不仅影响凋落物的化学性质,而且与分解环境中土壤的生物活性密切相关,但CO2浓度升高并不改变凋落物质量与分解速率之间的关系;越来越多的研究显示,CO2浓度升高的环境下,植物群落的物种组成会产生变化,这种变化对养分循环速率的影响远大于单纯大气CO2浓度变化的影响。     

高寒山区横坡绿肥聚垄免耕的效果与技术关键

高寒山区横坡绿肥聚垄免耕的效果与技术关键李明祥（贵州省六盘水市水土保持办公室，５５３００１）粮食的短缺和寻求摆脱粮食生产困境的路子是困扰高寒贫困山区生态建设和经济发展的一个历史性问题，是当前和今后一段时期山区治理开发中所面临的一项重要任务。出路何在？...     

贵溪冶炼厂周边农田土壤重金属污染特性及评价

在对贵溪冶炼厂周边区域的农田土壤及生产的稻谷进行采样调查和数据处理分析的基础上,对农田土壤重金属污染特性和现状进行了监测和初步评价,结果表明:农田土壤重金属污染可能来源于贵溪冶炼厂废水的排放及尾矿渣的堆放,与灌溉水源无关;用国家土壤环境质量二级标准进行评价可知,农田土壤重金属的综合污染指数较高,土壤污染已处于重度污染等级,单项重金属污染指数表明Cu、Cd的含量已严重超标,并处于重度污染等级,Zn、Pb和As的含量未构成污染;水泉村、竹山村与所生产的稻谷三者之间呈显著或极显著正相关,即农田生态系统呈复合污染的趋势。